DNA isolation procedures. pp. 249-287 In: DeSalle, R., Giribet, G. and Wheeler, W.C. (Eds.)

Authors: Nishiguchi, M.K., Doukakis, P., Egan, M., Goldstein, P.Z., Kizirian, D., Phillips, A., Prendini, L., Rosenbaum, H.C., Torres, E., Wyner, Y., and DeSalle, R. & Giribet, G

DOIPDF

Journal: Methods and Tools in Biosciences and Medicine. Techniques in Molecular Evolution and Systematics
Year: 2002
Published: January 1, 2002
Citation: Nishiguchi, M.K., Doukakis, P., Egan, M., Goldstein, P.Z., Kizirian, D., Phillips, A., Prendini, L., Rosenbaum, H.C., Torres, E., Wyner, Y., and DeSalle, R. & Giribet, G 2002. DNA isolation procedures. pp. 249-287 In: DeSalle, R., Giribet, G. and Wheeler, W.C. (Eds.). Methods and Tools in Biosciences and Medicine. Techniques in Molecular Evolution and Systematics. https://doi.org/10.1007/978-3-0348-8125-8_12

Abstract

Literally hundreds of protocols for DNA preparation from various sources of tissue have been published over the last few decades. To display all of these preparations would take volumes of manual space so instead we present in this chapter several of the preparations that have been used successfully in our laboratories. We also present a few “classical” procedures that are “tried and true” and nearly always work. In addition the www is an excellent source for protocols. Some forums exist for the dissemination of protocols for DNA and RNA isolation (DNA isolation protocols forums: http://www.nwfsc.noaa.gov/protocols.html , http://bric.postech.ac.kr/resources/rprotocol/ ; RNA isolation protocols forum: http://www.nwfsc.noaa.gov/protocols/methods/RNAMethodsMenu.html ).

Keywords and Taxa

DNA isolation procedures
Arachnida
DNA
Computer science